Introduction Metagenomics has transformed the study of microbial communities by enabling researchers to analyze DNA directly from environmental samples. Instead of isolating organisms in culture, sequencing environmental DNA allows …

Introduction Choosing the correct RNA-seq sequencing depth is one of the most important decisions when designing a transcriptomics experiment. Sequencing too few reads can reduce the ability to detect …

A practical guide to the bacterial genome assembly pipeline, from raw sequencing reads to polished assemblies and functional genome annotation.