Category: Bioinformatic Workflows
If you have assembled a bacterial or archaeal genome, the next question is usually straightforward: which annotation pipeline should you use? Three of the most widely used options are …
Introduction Shotgun metagenomics allows researchers to sequence all genetic material in an environmental sample. However, after assembly, the resulting dataset contains thousands of contigs from multiple organisms. To reconstruct …
Learn how to assemble a bacterial genome from Oxford Nanopore sequencing reads. This guide explains the complete genome assembly workflow using long-read sequencing data.
Introduction Metagenomics has transformed the study of microbial communities by enabling researchers to analyze DNA directly from environmental samples. Instead of isolating organisms in culture, sequencing environmental DNA allows …
Introduction Choosing the correct RNA-seq sequencing depth is one of the most important decisions when designing a transcriptomics experiment. Sequencing too few reads can reduce the ability to detect …
A practical guide to the bacterial genome assembly pipeline, from raw sequencing reads to polished assemblies and functional genome annotation.
A step-by-step guide to RNA-seq data analysis, from FASTQ quality control to differential gene expression and pathway enrichment.
Learn what is genome annotation, how it works, and which tools are most used. A complete guide to structural and functional genome annotation for beginners.
