A low RNA-seq mapping rate is one of the most common warning signs in transcriptomics analysis. If too many reads fail to align to the reference genome or transcriptome, …

Introduction Bacterial genomes vary widely in size depending on their ecology, lifestyle, and evolutionary history. Understanding the average bacterial genome size is essential for designing sequencing experiments, estimating coverage, …

Introduction Shotgun metagenomics allows researchers to sequence all genetic material in an environmental sample. However, after assembly, the resulting dataset contains thousands of contigs from multiple organisms. To reconstruct …