A step-by-step guide to RNA-seq data analysis, from FASTQ quality control to differential gene expression and pathway enrichment.
Introduction Microbial communities are extraordinarily complex. Even in apparently simple environments, bacteria, archaea, and microbial eukaryotes coexist in highly structured ecosystems where individual cells can differ dramatically in function, …
DNA sequencing has transformed modern biology, medicine, and biotechnology. From identifying disease-causing mutations to characterizing entire microbial communities, sequencing technologies are now at the core of life sciences. Two …