How to Interpret DESeq2 Results
Running DESeq2 is the straightforward part. Understanding what the output actually means — and avoiding the mistakes that lead to wrong conclusions — is where most researchers struggle. This …
Category: Transcriptomics
How to Interpret Differential Gene Expression Results
Differential gene expression analysis is one of the most common outputs of RNA-seq experiments. After running tools such as DESeq2, edgeR or limma-voom, researchers often receive a table containing …
Low RNA-seq Mapping Rate: Causes and Fixes
A low RNA-seq mapping rate is one of the most common warning signs in transcriptomics analysis. If too many reads fail to align to the reference genome or transcriptome, …
What Is Transcriptomics? How RNA-Seq Reveals What Microbes Are Doing
Explore transcriptomics, the study of RNA molecules that reveals which genes are active in cells and how they respond to their environment.