Latest Articles
Why Is My Metagenome Assembly So Fragmented? Common Causes and Fixes
Metagenome assembly is one of the most useful steps in shotgun metagenomics, but it is also one of the most…
Common Metagenomics Mistakes and How to Avoid Them
Metagenomics can generate powerful insights into microbial communities, from taxonomic composition to metabolic potential and genome recovery. But it is…
Prokka vs PGAP vs RAST: Which Annotation Pipeline Should You Use?
If you have assembled a bacterial or archaeal genome, the next question is usually straightforward: which annotation pipeline should you…
Low RNA-seq Mapping Rate: Causes and Fixes
A low RNA-seq mapping rate is one of the most common warning signs in transcriptomics analysis. If too many reads…
Single-Cell Sequencing in Microbiology: Unlocking Microbial Diversity One Cell at a Time
Introduction Microbial communities are extraordinarily complex. Even in apparently simple environments, bacteria, archaea, and microbial eukaryotes coexist in highly structured…
Next Generation Sequencing (NGS): Definition, Workflow, and Sanger vs NGS Comparison
DNA sequencing has transformed modern biology, medicine, and biotechnology. From identifying disease-causing mutations to characterizing entire microbial communities, sequencing technologies…
Shotgun Metagenomics Sequencing vs 16S rRNA Gene Sequencing
16S vs shotgun metagenomics: learn which sequencing method gives you taxonomic depth, functional insight and when to use each.
RNA-Seq Data Analysis Pipeline: From FASTQ Files to Differential Gene Expression
A step-by-step guide to RNA-seq data analysis, from FASTQ quality control to differential gene expression and pathway enrichment.
How Many Reads Do You Need for RNA-Seq? Sequencing Depth Explained
Introduction Choosing the correct RNA-seq sequencing depth is one of the most important decisions when designing a transcriptomics experiment. Sequencing…
What Is Genome Annotation? A Complete Guide to Understanding and Annotating Genomes
Learn what is genome annotation, how it works, and which tools are most used. A complete guide to structural and…